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Spider Strings is a Yellow tool that amplifies the effects of the Needolin in Hollow Knight: Silksong. Check out how to get Spider Strings, its location, and its effects here!
List of Contents

Spider Strings can be purchased for 320 Rosaries from Jubilana at Songclave. Make sure to gather enough Rosaries before buying or crafting this tool.

The Spider Strings only show up in Jubilana's wares after completing her second quest, The Lost Merchant. After accepting this quest, players will need to look for Jubilana in the Memorium. This place is only accessible with the Faydown Cloak.
The prerequisites to this quest are Building up Songclave and Strengthening Songclave. These quests require Hornet to donate 300 and 500 Rosaries respectively.
Strengthening Songclave Walkthrough

The Spider Strings strengthen the effect of the Needolin, increasing its range. While the Needolin is mainly used to open up doors such as the one in Weavenest Atla, using the Needolin can also stun enemies for a brief moment.

Playing the Needolin around the NPC's and enemies throughout Pharloom will allow Hornet to read their thoughts. While this serves no purpose, this gives insight into the story of Hollow Knight: Silksong.
I still have no idea what it does.. Is it that enemies will stay still for longer while playing music?